Thin Layer Chromatography(TLC) is a special application of adsorption chromatography. Here adsorbent is supported on a flat surface. In this chromatographic technique a thin layer of adsorbent is made on a glass surface specially over glass slides. Development of Chromatogram in this technique is accomplished by capillary movement of the solvent upon the thin layer of the adsorbent.
Fig: Schematic Representation of TLC.
Most common adsorbent used in TLC in order of importance are
Silica Gel > Alumina > Kieselghur > Cellulose
Common indicator used for developing process in TLC is iodine vapor. Here iodine vapor sprayed in the Chromatoplate after separation, is adsorbed in the area of plates containing organic compounds; brown spots are observed due to I2 charge transfer complex. Using TLC all most all compounds can be detected. This method is non destructive.
Fig: Schematic Representation of TLC.
Most common adsorbent used in TLC in order of importance are
Silica Gel > Alumina > Kieselghur > Cellulose
Common indicator used for developing process in TLC is iodine vapor. Here iodine vapor sprayed in the Chromatoplate after separation, is adsorbed in the area of plates containing organic compounds; brown spots are observed due to I2 charge transfer complex. Using TLC all most all compounds can be detected. This method is non destructive.
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